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4000-900-589

(1)

      ɸ߿Ƹɼg޹˾cRzºQRzHtCṩIzyգ䏊ɫͼɫС˵,Ҳlu,henhenshe123аlͶط߀ڽȡõĘsuP_Ōҡ͡пԺƌWԺɼDtWоءRzOzy߀ɞtԺЙC\gIṩn}о͌I\gա

 

(2)

Wg


1. SNP/ͻ׃zy
Q
      SNP͑ṩһĽQzyOӋOӋͺϳɡPCRUͼyԼ
`Č򞷽
      ҂ɸоĘӱĴСо^Ĺƌ򞷽SNPABI 3730XL DNAxӱMМyzymõĘӱǔʮǧSNPą^200ĉAǧA
Y߶Ⱦ_
      ˱C_Ⱥ͙z`ȣ҂ֻеĸ|^ڷõķܛзЧʲɫͼɫС˵,Ҳlu,henhenshe123CܙzyеĶλcsλc

(3)


Ճ
һ֪SNP/ͻ׃λczy
      ֪SNPλcMМy~횣VzԷ֪²Mл\ࡣ
Ҍδ֪SNP/ͻ׃λc
1. @Ӝy
      @Ӱϳɵ|ҪϢĺx@MМy򣬿԰lFԓµSNP/ͻ׃λcоcxPԵ춨ҪA
2. ȫy
      SNPλcλڻ{^tȺӺͻg^SNPλc܌ı_ˮƽһӰ푣ĶӰ푵|ı_cɫͼɫС˵,Ҳlu,henhenshe123PԠˣȫ򷶇MµSNP/ͻ׃λcьҲdzбҪ

(4)


ҪṩϢ
      zyϢSNPλcϢеȱϢ
      MDNA-20ȱѪҺͽM
SNPճZ
      ҂ṩٸ|ęzyYyDSNP漰Čȡ

 

2.ɹⶨPCR
      MĸͨABI 7900 Real-Time PCRxӱĿĻ_rMЙzy96װ壬384װTaqmanܶȱ_VоƬɝMɫͼɫС˵,Ҳlu,henhenshe123ͨͨĻ_SNPBԌ
Ճݣ
      Ŀǰ˾ṩɹⶨPCRzyķУEVAGREEN zyÿÿƷ؏ΣTaqmanzyÿÿƷ؏Σs̽ᘙzyÿÿƷ؏Ρ
ҪṩϢ
      ՈṩrIJϣֱṩõĿRNADNA5L/Ʒ
      ߀Ҫṩ֪ȫLУGene IDDNA/RNAԴSȵPԔYϡ
ճZ
      1.˾ṩԔĈ棬ṩăȫԝMՓĻnjµҪ
      2.˾CzyYʴ_^ţܴ_һҪõijضɫͼɫС˵,Ҳlu,henhenshe123Y

 

(5)


3. SSR&STR
      ҹ˾ͨ^ɹӛPCRU΢lλc^УABI 3730XL Analyserͬr96ƷğɹPCRaMëӾzy 30-2Сr֮΢lλc^дС΢lλcęzy͑ṩԔ΢lλcϢ
Ճݣ
      ҂ṩ͑PCRaDᘌ͑ضĘӱOӋ򞷽ɹӛxĺϳɣɹ Marker xõȣضӱSSR&STRͷա
ҪṩϢ
      ṩrֲϣɘӺҺكMDNAҪȸߣoȾ50 g
      ߀ҪṩԓĿPɹӛ͡
ճZ
      ҂ṩDVSTR/SSRͷYķ档

 

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4. ӿ¡
      ҹ˾ԞṩֱӷxMDNAMĿĻ¡ضmRNAD䛺ϳcDNAMп¡|DNAģā¡WϳĿĻMп¡PCRwUĿƬMп¡ȡ
Ճ
      TA¡͑ṩPCRaBӵpMD18-TdwУDYxy
      ¡ƬλԪƬΏһdwһdwͨ_dw
ҪṩϢ
      ṩrƷϻMDNA/RNAҪȸߣoȾ50 g
      ߀Ҫṩ֪ȫLУGene IDDNA/RNAԴSȵPԔYϡ
ճZ
      漰PCRڿ͑Cṩģ_ǰ£CɫͼɫС˵,Ҳlu,henhenshe123PCRUĻԪƬΟoƴaͻ׃A_99%ϣ
      ҂ṩoĿƬεL|ʹ̾dwԔϢyDбȌYĽY档
5. ȫϳ
Ճ
      ȫ˹ϳ
͑ҪṩϢ
      1. ϢĿĻл򵰰׵İлORFϢ
      2. dwQ
      3. Aڵ_ϵyϢ
      4. ĿĻɶ˵øλc
      5. ҪøλcԼҪøІc
ճZ
      ṩs4Lă|DNA1mLrҺЌļyԭʼDıļøλcDϳɈӔP

 

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6. ȺYԷ
      16S rDNAǼȾɫwϾa16S rRNADNAУ܉@ʾgͬȼˮƽϵخԣÿ׃^вͬ١NļMзbͨ^16S rDNAĎ򼚾16S rDNADGGE׃ݶzӾgԌijһضhмMз
Ճ
      16S rDNAĎ옋
      16S rDNAĎ죬xԿ¡y16S rDNAУcGenBank֪MͬԴԱ^@ضhеļȺYͼϢ
      DGGE׃ݶzӾ
      ضhƷлϼ16S rDNAͨ^׃ݶzӾ@ØƷеļȺYDVzӾlպ¡y򣬴_NPϵ@ضƷеļϢ
ҪṩϢ
      HṩrƷϻMDNAҪȸߣoȾ50 g
ճZ
      ҂ṩoDNAӾzyDyӱĜyDбȌYĽY档

 

(8)


ɫͼɫС˵,Ҳlu,henhenshe1237. Western BlottingӡE
      mVtWIˆTWߌWestern Blotting׌иĕrg;¿ƌWо˾ṩȫ׵Western Blottinggա
Ճ
      ȡ͑ṩĽMӱеĵףMSDS-PAGEӾDwSĤPVDFĤϣԓĤĿĵ׵خԿwM̎M@ɫ_ǷĿĵ׼ĴС
͑ҪṩϢ
      ṩrƷϻ򵰰טƷҪȸߣoȾ
      ߀ҪṩĿĵIDPԔYϡ
ճZ
      ֻṩrĽM򼚰Ʒ҂ɵȡȜyҪṩعXzƬxDƬČ档ԔČ򞷽ɫͼɫС˵,Ҳlu,henhenshe123ӡEYP

 

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